| Autor: |
Dilixiati Tuerdimaimaiti, Buzukela Abuduaini, Shaotao Kang, Jinliang Jiao, Mengchen Li, Wolazihan Madeniyati, Baihetinisha Tuerdi, Gulisitan Aili, Reyila Tuerhong, Ajiguli Kulaxi |
| Jazyk: |
angličtina |
| Rok vydání: |
2023 |
| Předmět: |
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| Zdroj: |
Journal of Inflammation, Vol 20, Iss 1, Pp 1-12 (2023) |
| Druh dokumentu: |
article |
| ISSN: |
1476-9255 |
| DOI: |
10.1186/s12950-023-00355-w |
| Popis: |
Abstract Background An increasing body of evidence now shows that the long-term mortality of patients with sepsis are associated with various sepsis-related immune cell defects. Alternative splicing (AS), as a sepsis-related immune cell defect, is considered as a potential immunomodulatory therapy target to improve patient outcomes. However, our understanding of the role AS plays in sepsis is currently insufficient. Aim This study investigated possible associations between AS and the gene regulatory networks affecting immune cells. We also investigated apoptosis and AS functionality in sepsis pathophysiology. Methods In this study, we assessed publicly available mRNA-seq data that was obtained from the NCBI GEO dataset (GSE154918), which included a healthy group (HLTY), a mild infection group (INF1), asepsis group (Seps), and a septic shock group (Shock). A total of 79 samples (excluding significant outliers) were identified by a poly-A capture method to generate RNA-seq data. The variable splicing events and highly correlated RNA binding protein (RBP) genes in each group were then systematically analyzed. Results For the first time, we used systematic RNA-seq analysis of sepsis-related AS and identified 1505 variable AS events that differed significantly (p |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
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