Comparative Analysis of Biological Properties of Large-Scale Expanded Adult Neural Crest-Derived Stem Cells Isolated from Human Hair Follicle and Skin Dermis

Autor: Roman G. Vasyliev, Olga S. Gubar, Inna M. Gordiienko, Larisa S. Litvinova, Anzhela E. Rodnichenko, Valeria V. Shupletsova, Alona V. Zlatska, Kristina A. Yurova, Natalia M. Todosenko, Veronika E. Khadzhynova, Mariia V. Shulha, Svitlana N. Novikova, Dmytro O. Zubov
Jazyk: angličtina
Rok vydání: 2019
Předmět:
Zdroj: Stem Cells International, Vol 2019 (2019)
Druh dokumentu: article
ISSN: 1687-966X
1687-9678
DOI: 10.1155/2019/9640790
Popis: Introduction. The adult neural crest-derived stem cells (NCSCs) have significant perspectives for use in regenerative medicine. The most attractive sources for adult NCSC isolation are the hair follicles (HF) and skin dermis (SD) because of easy access and minimally invasive biopsy. The aim of this study was to compare the biological properties of HF- and SD-derived NCSCs after their large-scale expansion. Methods. The conventional explant method was used to obtain HF NCSCs. For the isolation of SD NCSCs, a new combined technique consisting of preplating and subsequent culturing in 3D blood plasma-derived fibrin hydrogel was applied. The studied cells were characterized by flow cytometry, ICC, qPCR, Bio-Plex multiplex assay, and directed multilineage differentiation assays. Results. We have obtained both adult SD and HF NCSCs from each skin sample (n=5). Adult SD and HF NCSCs were positive for key neural crest markers: SOX10, P75 (CD271), NESTIN, SOX2, and CD349. SD NCSCs showed a higher growth rate during the large-scale expansion compared to HF NCSCs (p
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