The effect of levosimendan on myocardial ischemia–reperfusion injury in streptozotocin-induced diabetic rats

Autor: Hasan Ali Kiraz, Fatih Poyraz, Gülay Kip, Özlem Erdem, Metin Alkan, Mustafa Arslan, Abdullah Özer, Volkan Şivgin, Faruk Metin Çomu
Jazyk: angličtina
Rok vydání: 2015
Předmět:
Zdroj: Libyan Journal of Medicine, Vol 10, Iss 0, Pp 1-6 (2015)
Druh dokumentu: article
ISSN: 1819-6357
DOI: 10.3402/ljm.v10.29269
Popis: Objective: Ischemia/reperfusion (I/R) injury is an important cause of myocardial damage by means of oxidative, inflammatory, and apoptotic mechanisms. The aim of the present study was to examine the potential cardio protective effects of levosimendan in a diabetic rat model of myocardial I/R injury. Methods: A total of 18 streptozotocin-induced diabetic Wistar Albino rats (55 mg/kg) were randomly divided into three equal groups as follows: the diabetic I/R group (DIR) in which myocardial I/R was induced following left thoracotomy, by ligating the left anterior descending coronary artery for 60 min, followed by 2 h of reperfusion; the diabetic I/R levosimendan group (DIRL), which underwent I/R by the same method while taking levosimendan intraperitoneal 12 µg kg−1; and the diabetic control group (DC) which underwent sham operations without tightening of the coronary sutures. As a control group (C), six healthy age-matched Wistar Albino rats underwent sham operations similar to the DC group. Two hours after the operation, the rats were sacrificed and the myocardial tissue samples were examined by light microscopy for evidence of myonecrosis and inflammatory cell infiltration. Results: Myonecrosis findings were significantly different among groups (p=0.008). Myonecrosis was more pronounced in the DIR group compared with the C, DC, and DIRL groups (p=0.001, p=0.007 and p=0.037, respectively). Similarly, the degree of inflammatory cell infiltration showed significant difference among groups (p
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