Autor: |
Ke Li, Ming Yang, Mengyue Tian, Li Jia, Yinghao Wu, Jinliang Du, Lining Yuan, Lianmin Li, Yuzhong Ma |
Jazyk: |
angličtina |
Rok vydání: |
2024 |
Předmět: |
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Zdroj: |
Journal of Inflammation, Vol 21, Iss 1, Pp 1-9 (2024) |
Druh dokumentu: |
article |
ISSN: |
1476-9255 |
DOI: |
10.1186/s12950-024-00378-x |
Popis: |
Abstract Background Lactobacillus casei possesses many kinds of bioactivities, such as anti-inflammation and anti-oxidant, and has been applied to treating multiple inflammatory diseases. However, its role in mastitis prevention has remained ambiguous. Methods This study aimed to examine the mechanisms underlying the preventive effects of L. casei 03 against E. coli- mastitis utilizing bovine mammary epithelial cells (BMECs) and a mouse model. Results In vitro assays revealed pretreatment with L. casei 03 reduced the apoptotic ratio and the mRNA expression levels of IL1β, IL6 and TNFα and suppressed phosphorylation of p65, IκBα, p38, JNK and ERK in the NF-κB signaling pathway and MAPK signaling pathway. Furthermore, in vivo tests indicated that intramammary infusion of L. casei 03 relieved pathological changes, reduced the secretion of IL1β, IL6 and TNFα and MPO activity in the mouse mastitis model. Conclusions These data suggest that L. casei 03 exerts protective effects against E. coli-induced mastitis in vitro and in vivo and may hold promise as a novel agent for the prevention and treatment of mastitis. |
Databáze: |
Directory of Open Access Journals |
Externí odkaz: |
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