Monitoring G protein-coupled receptor and β-arrestin trafficking in live cells using enhanced bystander BRET

Autor: Yoon Namkung, Christian Le Gouill, Viktoria Lukashova, Hiroyuki Kobayashi, Mireille Hogue, Etienne Khoury, Mideum Song, Michel Bouvier, Stéphane A. Laporte
Jazyk: angličtina
Rok vydání: 2016
Předmět:
Zdroj: Nature Communications, Vol 7, Iss 1, Pp 1-12 (2016)
Druh dokumentu: article
ISSN: 2041-1723
DOI: 10.1038/ncomms12178
Popis: Cellular signaling processes often involve trafficking of receptors and other proteins between subcellular compartments. Here the authors demonstrate a method based on the concept of Enhanced bystander Bioluminescence Resonance Energy Transfer (EbBRET) that allows efficient real time monitoring of endocytosis and trafficking.
Databáze: Directory of Open Access Journals