REAL TIME POLYMERASE CHAIN REACTION IN TULAREMIA LABORATORY DIAGNOSTICS
| Autor: | M. I Kormilitsyna, I. S Mescheryakova, T. V Mikhailova, A. A Dobrovolsky |
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| Jazyk: | ruština |
| Rok vydání: | 2015 |
| Předmět: | |
| Zdroj: | Журнал микробиологии, эпидемиологии и иммунобиологии, Vol 92, Iss 3, Pp 59-63 (2015) |
| Druh dokumentu: | article |
| ISSN: | 0372-9311 2686-7613 |
| Popis: | Aim. Enhancement of tularemia laboratory diagnostics by F. tularensis DNA determination in blood sera of patients using real time polymerase chain reaction (RT-PCR). Materials and methods. 39 blood sera of patients obtained during transmissive epidemic outbreak of tularemia in Khanty-Mansiysk in 2013 were studied in agglutination reaction, passive hemagglutination, RT-PCR. Specific primers and fluorescent probes were used: ISFTu2F/R+ISFTu2P, Tul4GF/R+tul4-PR2. Results. Advantages of using RT-PCR for early diagnostics of tularemia, when specific antibodies are not detected using traditional immunologic methods, were established. Use of a combination of primers and ISFTu2F/R+ISFTu2P probe allowed to detect F. tularensis DNA in 100% of sera, whereas Tul4GF/R+tul4-PR2 combination - 92% ofsera. The data were obtained when DNA was isolated from sera using «Proba Rapid» express method. Clinical-epidemiologic diagnosis oftularemia was confirmed by both immune-serologic and RT-PCR methods when sera were studied 3 - 4 weeks after the onset of the disease. Conclusion. RT-PCR with ISFTu2F/R primers and fluorescent probe ISFTu2P, having high sensitivity and specificity, allows to determine F. tularensis DNA in blood sera of patients at both the early stage and 3 - 4 weeks after the onset of the disease. |
| Databáze: | Directory of Open Access Journals |
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