Comprehensive RNA sequencing in primary murine keratinocytes and fibroblasts identifies novel biomarkers and provides potential therapeutic targets for skin-related diseases

Autor: Tiancheng Wang, Zhenwei Zhou, Enjing Luo, Jinghong Zhong, Daqing Zhao, Haisi Dong, Baojin Yao
Jazyk: angličtina
Rok vydání: 2021
Předmět:
Zdroj: Cellular & Molecular Biology Letters, Vol 26, Iss 1, Pp 1-15 (2021)
Druh dokumentu: article
ISSN: 1425-8153
1689-1392
53164792
DOI: 10.1186/s11658-021-00285-6
Popis: Abstract Background Keratinocytes and fibroblasts represent the major cell types in the epidermis and dermis of the skin and play a significant role in maintenance of skin homeostasis. However, the biological characteristics of keratinocytes and fibroblasts remain to be elucidated. The purpose of this study was to compare the gene expression pattern between keratinocytes and fibroblasts and to explore novel biomarker genes so as to provide potential therapeutic targets for skin-related diseases such as burns, wounds, and aging. Methods Skin keratinocytes and fibroblasts were isolated from newborn mice. To fully understand the heterogeneity of gene expression between keratinocytes and fibroblasts, differentially expressed genes (DEGs) between the two cell types were detected by RNA-seq technology. Quantitative real-time polymerase chain reaction (qRT-PCR) was used to detect the known genes of keratinocytes and fibroblasts and verify the RNA-seq results. Results Transcriptomic data showed a total of 4309 DEGs (fold-change > 1.5 and q-value
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