Fine mapping of powdery mildew resistance gene PmTm4 in wheat using comparative genomics

Autor: Jing-zhong XIE, Li-li WANG, Yong WANG, Huai-zhi ZHANG, Sheng-hui ZHOU, Qiu-hong WU, Yong-xing CHEN, Zhen-zhong WANG, Guo-xin WANG, De-yun ZHANG, Yan ZHANG, Tie-zhu HU, Zhi-yong LIU
Jazyk: angličtina
Rok vydání: 2017
Předmět:
Zdroj: Journal of Integrative Agriculture, Vol 16, Iss 3, Pp 540-550 (2017)
Druh dokumentu: article
ISSN: 2095-3119
DOI: 10.1016/S2095-3119(16)61377-1
Popis: Powdery mildew, caused by Blumeria graminis f. sp. tritici, is one of the most severe wheat diseases. Mining powdery mildew resistance genes in wheat cultivars and their appliance in breeding program is a promising way to control this disease. Genetic analysis revealed that a single dominant resistance gene named PmTm4 originated from Chinese wheat line Tangmai 4 confers resistance to prevailing isolates of B. graminis f. sp. tritici isolate E09. Detailed comparative genomics analyses helped to develop closely linked markers to PmTm4 and a fine genetic map was constructed using large F2 population, in which PmTm4 was located into a 0.66-cM genetic interval. The orthologous subgenome region of PmTm4 in Aegilops tauschii was identified, and two resistance gene analogs (RGA) were characterized from the corresponding sequence scaffolds of Ae. tauschii draft assembly. The closely linked markers and identified Ae. tauschii orthologs in the mapping interval provide an entry point for chromosome landing and map-based cloning of PmTm4.
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