| Autor: |
Thomas B. Layton, Lynn Williams, Huw Colin-York, Fiona E. McCann, Marisa Cabrita, Marc Feldmann, Cameron Brown, Weilin Xie, Marco Fritzsche, Dominic Furniss, Jagdeep Nanchahal |
| Jazyk: |
angličtina |
| Rok vydání: |
2020 |
| Předmět: |
|
| Zdroj: |
Biology Open, Vol 9, Iss 3 (2020) |
| Druh dokumentu: |
article |
| ISSN: |
2046-6390 |
| DOI: |
10.1242/bio.049809 |
| Popis: |
Mechanical force is a fundamental regulator of cell phenotype. Myofibroblasts are central mediators of fibrosis, a major unmet clinical need characterised by the deposition of excessive matrix proteins. Traction forces of myofibroblasts play a key role in remodelling the matrix and modulate the activities of embedded stromal cells. Here, we employ a combination of unsupervised computational analysis, cytoskeletal profiling and single cell traction force microscopy as a functional readout to uncover how the complex spatiotemporal dynamics and mechanics of living human myofibroblast shape sub-cellular profiling of traction forces in fibrosis. We resolve distinct biophysical communities of myofibroblasts, and our results provide a new paradigm for studying functional heterogeneity in human stromal cells. |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
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