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为利用克里藻JNU41高效生产油脂、亚油酸和叶黄素等重要代谢产物，对其进行培养条件的优化及代谢产物积累的评估。采用改良的BG-11（mBG-11）和BBM（mBBM）培养基，设置3组光照强度〔单侧100 μmol/(m2·s)、单侧300 μmol/(m2·s)和双侧300 μmol/（m2·s）〕和6种初始氮浓度（3、6、9、12、15、18 mmol/L）对克里藻JNU41进行培养，并测定其生长曲线、生化组成、总脂产率、脂肪酸组成及叶黄素含量。结果表明：克里藻JNU41在mBBM培养基、双侧光照强度300 μmol/（m2·s）、初始氮浓度6 mmol/L条件下，生物量和总脂产率最高,分别为8.38 g/L和281.71 mg/(L·d)；在mBG-11培养基、双侧光照强度300 μmol/(m2·s)、初始氮浓度3 mmol/L条件下，总脂和总脂肪酸含量最高，分别为5512%和47.10%。克里藻JNU41主要脂肪酸组成为棕榈酸、油酸、亚油酸和亚麻酸，其中亚油酸含量最高，亚油酸最高相对含量为65.87%。此外，克里藻JNU41还积累一定量叶黄素，最高含量可达1.64 mg/g。因此，克里藻JNU41是一株可用于生产多种高附加值产物的潜力藻株。To efficient production of important metabolites such as oil, linoleic acid and lutein by Klebsormidium sp. JNU41, the culture conditions were optimized and the accumulation of metabolites in Klebsormidium sp. JNU41 was evaluated. The growth curve, biochemical composition, total lipid yield, fatty acid composition and lutein content of Klebsormidium sp. JNU41 were studied using modified BG-11 (mBG-11) and BBM (mBBM) as the media, and three sets of light intensities 〔unilateral 100 μmol/(m2·s), unilateral 300 μmol/(m2·s) and bilateral 300 μmol/(m2·s)〕 and six initial nitrogen concentrations (3, 6, 9, 12, 15, 18 mmol/L) were used for culture. The results showed that under the conditions of mBBM media, bilateral light intensity 300 μmol/(m2·s) and initial nitrogen concentration 6 mmol/L, the biomass (8.38g/L) and total lipid yield 〔281.71 mg/(L·d)〕 reached a maximum value. Under the conditions of mBG-11 media, bilateral light intensity 300 μmol/(m2·s) and initial nitrogen concentration 3 mmol/L, the highest total lipid and total fatty acid contents reached 55.12% and 47.10%, respectively. The main components of fatty acids were palmitic acid, oleic acid, linoleic acid and linolenic acid, and linoleic acid content was the highest with the highest relative content of 65.87%. In addition, Klebsormidium sp. JNU41 also accumulated a certain amount of lutein, up to 1.64 mg/g. Therefore, Klebsormidium sp. JNU41 is an algal strain that can be used to produce high value-added products. |
