Transcriptome-based analysis of salt-related genes during the sprout stage of common bean (Phaseolus vulgaris) under salt stress conditions

Autor: Qi Zhang, Ming Li, Chun Yang Xia, Wen Jing Zhang, Zhen Gong Yin, You Li Zhang, Qing Xi Fang, Yang Cheng Liu, Ming Yu Zhang, Wen Hui Zhang, Ji Dao Du, Yan Li Du
Jazyk: angličtina
Rok vydání: 2021
Předmět:
Zdroj: Biotechnology & Biotechnological Equipment, Vol 35, Iss 1, Pp 1086-1098 (2021)
Druh dokumentu: article
ISSN: 1310-2818
1314-3530
13102818
DOI: 10.1080/13102818.2021.1954091
Popis: Salt stress is an important factor restricting crop growth. Thus, genes related to plant responses to high salinity at the sprout stage of common bean (Phaseolus vulgaris) should be identified and characterized. In this study, on the basis of the common bean germination index, 0.4% NaCl and day 5 of the incubation period were selected as the optimal salt concentration and sampling time, respectively. Salt-tolerant and salt-sensitive plant materials suitable for the local region were selected as representative common bean materials with contrasting salt-related phenotypes. A total of 441 differentially expressed genes (DEGs) were identified by analyzing the generated RNA-seq data. The enriched Gene Ontology terms and Kyoto Encyclopedia of Genes and Genomes pathways indicated the DEGs were associated with oxidoreductase activities and phenylpropanoid biosynthesis. Furthermore, the superoxide dismutase, peroxidase, and catalase activities and the lignin content, which are related to oxidoreductase activities and phenylpropanoid biosynthesis, were revealed to be significantly associated with common bean salt tolerance. The six DEGs with salt tolerance-related Arabidopsis thaliana homologs may be important candidate genes mediating the salt stress responses of common bean during the sprout stage. Supplemental data for this article is available online at https://doi.org/10.1080/13102818.2021.1954091 .
Databáze: Directory of Open Access Journals
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