Characterization of type and genetic diversity among soybean cyst nematode differentiators

Autor: Éder Matsuo, Tuneo Sediyama, Rosângela D'Arc de Lima Oliveira, Cosme Damião Cruz, Rita de Cássia Teixeira Oliveira
Jazyk: English<br />Spanish; Castilian<br />Portuguese
Rok vydání: 2012
Předmět:
Zdroj: Scientia Agricola, Vol 69, Iss 2, Pp 147-151 (2012)
Druh dokumentu: article
ISSN: 0103-9016
1678-992X
DOI: 10.1590/S0103-90162012000200010
Popis: The development of soybean cyst nematode, Heterodera glycines Ichinohe, resistant genotypes with high yields has been one of the objectives of soybean (Glycine max (L.) Merrill) breeding programs. The objective of this study was to characterize the pathotype of soybean cyst nematodes and analyze the genetic diversity of ten differentiator lines ('Lee 74', Peking, Pickett, PI 88788, PI 90763, PI 437654, PI 209332, PI 89772, PI 548316 and 'Hartwig'). Inoculum was obtained from plants cultivated in field soil in Viçosa, state of Minas Gerais, Brazil. Thirty-four days after inoculating each plant with 4,000 eggs, the number of females, female index, total number of eggs, number of eggs per female, reproduction factor, plant height, number of nodes, fresh and dry matter weights were assessed. The differential lines were first grouped with Scott-Knott test. Subsequently, the genetic diversity was evaluated using dendrograms, graphic analysis and the Tocher grouping method. The inoculum of H. glycines obtained from NBSGBP-UFV was characterized as HG Type 0. The differentiating lines were divergent, and PI 89772, PI 437654, 'Hartwig' and 'Peking' had the greatest potential for use in breeding programs.
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