| Autor: |
Wangchao Yao, Hongbing Gu, Tao Hong, Yao Wang, Sihao Chen, Xiumei Mo, Wenyao Li, Chunsheng Wang, Tonghe Zhu, Shuyang Lu |
| Jazyk: |
angličtina |
| Rok vydání: |
2020 |
| Předmět: |
|
| Zdroj: |
Materials & Design, Vol 194, Iss , Pp 108943- (2020) |
| Druh dokumentu: |
article |
| ISSN: |
0264-1275 |
| DOI: |
10.1016/j.matdes.2020.108943 |
| Popis: |
Acute coagulation is one of the vexed problems in transplantation of small-diameter artificial blood vessel. Three-dimensional porous heparin-modified gelatin (Gel)@chitosan (CS) tubular scaffold were successfully acquired by using the method of freeze-drying combined with amination for application in tissue regeneration of blood vessels. Initially, homogeneous gelatin solution was initially poured into a tubular mold and underwent a procedure of vacuum freeze-drying to form a three-dimensional porous tubular skeleton. Chitosan was used to loading heparin (Hep) which is a kind of efficient anticoagulant. The Hep-loaded CS composite solution were poured into Gel tubular skeleton, following freeze-drying matched EDC-NHS crosslinking to form Gel@CS-Hep tubular scaffold exhibited a three-dimensional structure and porous morphology. Then, poly(ester-urethane)urea/gelatin (PU75) micro-nano fibers were electrospinning outside the Gel@CS-Hep tube as mechanical reinforcement layer. The Gel@CS-Hep/PU75 tube showed higher hydrophilicity, stable mechanical properties as well as no cytotoxicity on human umbilical vein endothelial cells. Importantly, the three-dimensional functional Gel@CS-Hep/PU75 tubular scaffold shows a good rapid endothelialization performance and effective anti-acute coagulation properties. Therefore, the developed Gel@CS-Hep/PU75 tube was proposed to be a potential scaffold for remodeling vascular tissue. |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
|
Full Text from ScienceDirect