Effects of PM2.5 sub-chronic exposure on liver metabolomics in mice
| Autor: | Liu YANG, Siqi DOU, Xinyuan LI, Shuo WEN, Kun PAN, Biao WU, Jinzhuo ZHAO, Jianjun XU, Peng LYU |
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| Jazyk: | English<br />Chinese |
| Rok vydání: | 2024 |
| Předmět: | |
| Zdroj: | 环境与职业医学, Vol 41, Iss 2, Pp 207-213 (2024) |
| Druh dokumentu: | article |
| ISSN: | 2095-9982 32808968 |
| DOI: | 10.11836/JEOM23224 |
| Popis: | BackgroundAtmospheric fine particulate matter (PM2.5) can disrupt the metabolic homeostasis of the liver and accelerate the progression of liver diseases, but there are few studies on the effects of sub-chronic PM2.5 exposure on the liver metabolome. ObjectivesTo investigate the effects of sub-chronic exposure to concentrated PM2.5 on hepatic metabolomics in mice by liquid chromatography-mass spectrometry (LC-MS), and to identify potentially affected metabolites and metabolic pathways. MethodsTwelve male C57BL/6J (6 weeks old) mice were randomly divided into two groups: a concentrated PM2.5 exposure group and a clean air exposure group. The mice were exposed to concentrated PM2.5 using the "Shanghai Meteorological and Environmental Animal Exposure System" at Fudan University. The exposure duration was 8 h per day, 6 d per week, for a total of 8 weeks. The mice's liver tissues were collected 24 h after the completion of exposure. LC-MS was performed to assess changes in the hepatic metabolome. Orthogonal partial least squares discriminant analysis and t-test were employed to identify differentially regulated metabolites between the two groups under the conditions of variable important in projection (VIP)≥1.0 and P |
| Databáze: | Directory of Open Access Journals |
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