Protective effects of upregulated HO-1 gene against the apoptosis of human retinal pigment epithelial cells in vitro

Autor: Yu Hong, Yu-Ping Liang, Wei-Qi Chen, Liu-Xia You, Qing-Feng Ni, Xiu-Yun Gao, Xiao-Rong Lin
Jazyk: angličtina
Rok vydání: 2021
Předmět:
Zdroj: International Journal of Ophthalmology, Vol 14, Iss 5, Pp 649-655 (2021)
Druh dokumentu: article
ISSN: 2222-3959
2227-4898
DOI: 10.18240/ijo.2021.05.03
Popis: AIM: To investigate the protective effect of heme oxygenase-1 (HO-1) against H2O2-induced apoptosis in human ARPE-19 cells. METHODS: The lentiviral vector expressing HO-1 was prepared and transfected into apoptotic ARPE-19 cells induced by H2O2. Functional experiments including cell counting kit-8 (CCK-8) assay, flow cytometry (FCM) and mitochondrial membrane potential assay were conducted. RESULTS: The ultrastructure of ARPE-19 cells was observed using transmission electron microscope (TEM). It was found that exogenous HO-1 significantly ameliorated H2O2-induced loss of cell viability, apoptosis and intracellular levels of reactive oxygen species (ROS) in ARPE-19 cells. The overexpression of HO-1 facilitated the transfer of nuclear factor erythroid-2-related factor 2 (Nrf2) from cytoplasm to nucleus, which in turn upregualted expressions HO-1 and B-cell lymphoma-2 (Bcl-2). Furthermore, HO-1 upregulation further inhibited H2O2-induced release of cysteinyl aspartate specific proteinase-3 (caspase-3). CONCLUSION: Exogenous HO-1 protect ARPE-19 cells against H2O2-induced oxidative stress by regulating the expressions of Nrf2, HO-1, Bcl-2, and caspase-3.
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