Identification, mapping and relative quantitation of SARS-CoV-2 Spike glycopeptides by Mass-Retention Time Fingerprinting

Autor: Rod Chalk, William E. P. Greenland, Tiago Moreira, Jesse Coker, Shubhashish M. M. Mukhopadhyay, Eleanor Williams, Charlotte Manning, Tina Bohstedt, Rama McCrorie, Alejandra Fernandez-Cid, Nicola A. Burgess-Brown
Jazyk: angličtina
Rok vydání: 2021
Předmět:
Zdroj: Communications Biology, Vol 4, Iss 1, Pp 1-8 (2021)
Druh dokumentu: article
ISSN: 2399-3642
DOI: 10.1038/s42003-021-02455-w
Popis: Mapping multiple glycan species to the same peptide and location from shared retention time of glycopeptides with MS and HPLC, Chalk, Greenland et al. construct a detailed mass-retention time database for Spike protein glycopeptides. This allows any mass LC-MS laboratory to reliably identify and quantify Spike protein glycopeptides from a single overnight elastase protein digest in less than 90 minutes.
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