| Autor: |
WANG Yuwen, LI Furong, YUAN Rongdi |
| Jazyk: |
čínština |
| Rok vydání: |
2024 |
| Předmět: |
|
| Zdroj: |
陆军军医大学学报, Vol 46, Iss 18, Pp 2101-2109 (2024) |
| Druh dokumentu: |
article |
| ISSN: |
2097-0927 |
| DOI: |
10.16016/j.2097-0927.202403058 |
| Popis: |
Objective To investigate the changes in G protein-coupled receptor 124 (Gpr124) expression in human retinal microvascular endothelial cells (HRMEC) under high-glucose condition and the regulatory effect of Gpr124 interference on HRMEC. Methods Immunofluorescence assay was used to observe the expression of Gpr124 in HRMEC. The cells were divided into a blank group and a high glucose group. CCK-8 assay and EdU cell proliferation assay were used to detect cell viability and proliferation, respectively. The Gpr124 expression was knocked down by transducting lentiviral virus carrying shRNA targeting Gpr124. Thus, the cells were divided into a blank control group, a high glucose control group, a high glucose+shRNA group, and a high glucose+Gpr124 shRNA group. Cell scratch test was performed to evaluate cell migration, and Matrigel plug assay was employed to assess the cell tubule formation. Western blotting was applied to detect the protein levels of Gpr124, VEGFA, MMP9, and β-catenin. Results The HRMEC from the high glucose group showed enhanced cell viability and increased number of proliferating cells compared to the cells of the blank group (P |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
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