Dideoxy single allele-specific PCR - DSASP new method to discrimination allelic
| Autor: | Eleonidas Moura Lima, Otávio Sérgio Lopes, Leonardo Ferreira Soares, Talitta Dantas Arruda, Carolina Oliveira Gigek, Cynthia Germoglio Farias Melo, Marilia Arruda Cardoso Smith, João Ricardo Gonçalves Oliveira, Arnaldo Medeiros, Plínio Delatorre, Rommel Rodriguez Burbano |
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| Jazyk: | angličtina |
| Rok vydání: | 2015 |
| Předmět: | |
| Zdroj: | Brazilian Archives of Biology and Technology, Vol 58, Iss 3, Pp 414-420 (2015) |
| Druh dokumentu: | article |
| ISSN: | 1678-4324 1516-8913 |
| DOI: | 10.1590/S1516-8913201500434 |
| Popis: | Gastric cancer (GC) is a multifactorial disease with a high mortality rate in Brazil and worldwide. This work aimed to evaluate single nucleotide polymorphisms (SNP) rs1695, in the Glutathione S-Transferase Pi (GSTP1) gene in GC samples by comparative analysis Specific PCR - ASP and Dideoxy Single Allele-Specific PCR - DSASP methods. The DSASP is the proposed new method for allelic discrimination. This work analyzed 60 GC samples, 26 diffuse and 34 intestinal types. The SNP rs1695 of the GSTP1 gene was significantly associated with GC analyzed by DSASP method (χ2 = 9.7, P < 0.05). A comparative analysis of the data obtained from both methods did not differ significantly (χ2 = 0.08, P > 0.05). These results suggest that the SNP rs1695 of the GSTP1 gene was a risk factor associated with gastric carcinogens is and the DSASP method was a new successfully low-cost strategy to study allelic discrimination. |
| Databáze: | Directory of Open Access Journals |
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