Microwave Interferometric Cytometry for Signal Analysis of Single Yeast Cells

Autor: Meng Zhang, Guangxin Huo, Juncheng Bao, Tomislav Markovic, Patrick Van Dijck, Bart Nauwelaers
Jazyk: angličtina
Rok vydání: 2022
Předmět:
Zdroj: Chemosensors, Vol 10, Iss 8, p 318 (2022)
Druh dokumentu: article
ISSN: 2227-9040
DOI: 10.3390/chemosensors10080318
Popis: Microwave dielectric sensing offers a rapid, label-free, and non-invasive way of characterization and sensing of biological materials at the microfluidic scale. In this work, a dielectric sensing is achieved with a microwave interferometric setup that is applied to cytometric applications. A fast way to analyze and design an interferometric system at microwave frequencies in software tools is proposed together with a novel manufacturing and assembly process, which enables a short recovery time and avoids extensive microwave-microfluidic chip fabrication. The simulation and measurement results of the interferometric setup are in agreement with an excellent match at levels below S21 = −60 dB. The sensitive microwave setup is evaluated on measurements of 3 µm polystyrene spheres and finally applied for characterization of a widely used laboratory Saccharomyces cerevisiae strain, the S288C, in a frequency range from 4 to 18 GHz.
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