Fluorescent molecular rotors as versatile in situ sensors for protein quantitation

Autor:	Kevin Daus, Sorachat Tharamak, Wanchai Pluempanupat, Peter A. Galie, Maria A. Theodoraki, Emmanuel A. Theodorakis, Mary L. Alpaugh
Jazyk:	angličtina
Rok vydání:	2023
Předmět:	Medicine Science
Zdroj:	Scientific Reports, Vol 13, Iss 1, Pp 1-12 (2023)
Druh dokumentu:	article
ISSN:	2045-2322
DOI:	10.1038/s41598-023-46571-5
Popis:	Abstract Accurate protein quantitation is essential for many cellular mechanistic studies. Existing technology relies on extrinsic sample evaluation that requires significant volumes of sample as well as addition of assay-specific reagents and importantly, is a terminal analysis. This study exploits the unique chemical features of a fluorescent molecular rotor that fluctuates between twisted-to-untwisted states, with a subsequent intensity increase in fluorescence depending on environmental conditions (e.g., viscosity). Here we report the development of a rapid, sensitive in situ protein quantitation method using ARCAM-1, a representative fluorescent molecular rotor that can be employed in both non-terminal and terminal assays.
Databáze:	Directory of Open Access Journals
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