Metal Organic Frame-Upconverting Nanoparticle Assemblies for the FRET Based Sensor Detection of Bisphenol A in High-Salt Foods

Autor: Zhou Xu, Lin-wei Zhang, Ling-li Long, Shao-hua Zhu, Mao-long Chen, Li Ding, Yun-hui Cheng
Jazyk: angličtina
Rok vydání: 2020
Předmět:
Zdroj: Frontiers in Bioengineering and Biotechnology, Vol 8 (2020)
Druh dokumentu: article
ISSN: 2296-4185
DOI: 10.3389/fbioe.2020.626269
Popis: To resolve the occurrence of unfulfillable detection in high-salts foods, we used fluorescence resonant energy transfer (FRET) sensors based on nanoparticle upconversion. In this study, we developed a novel FRET sensor for the detection of bisphenol A (BPA) in high-salt foods. We based this approach on the assembly of aptamer modified upconversion nanoparticles (DNA1-UCNPs) and complementary DNA modified metal organic frames (DNA2-MOFs), which possessed corresponding wavelength absorption. Targeting BPA signal transduction was performed using the BPA aptamer, via competitive recognition between the BPA analyte and complementary DNA sequences in a high-salt solution. Sensor adaption in high-salt samples was attributed to functional hydrophilic groups, modified in the MOFs, and the enhanced colloidal stability of these MOFs. The MOF-UCNP assembly displayed considerable analytical performance in terms of BPA detection, with a linear range of 0.1–100 nM, and a limit of detection (LOD) of 0.02 nM, in a 340 mM NaCl food sample (the energy drink, Gatorade). Thus, this method provides a solid basis for small molecules detection in high-salt foods.
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