Serodiagnosis of Oesophagostomum Columbianum infection in Goats using indirect ELISA
| Autor: | Fayaz Ahmad, M. Z. Chisti, Bashir Ahmad Lone, Hidayatullah Tak |
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| Jazyk: | angličtina |
| Rok vydání: | 2011 |
| Předmět: | |
| Zdroj: | Veterinary World, Vol 4, Iss 11.000, Pp 503-506 (2011) |
| Druh dokumentu: | article |
| ISSN: | 0972-8988 2231-0916 |
| Popis: | To determine the seroprevalence of Oesophagostomum columbianum antibodies in goats in Kashmnir, a study was conducted in Srinagar and Ganderbal Districts, located in central parts of Kashmir valley. ELISA was standardized and evaluated to detect goat Oesophagostomosis in experimental and clinical cases using somatic whole adult antigen of Oesophagostomum columbianum. Plate ELISA was standardized using 5μg/well antigen concentration with 1:100 and 1:1000 of sera and conjugate dilution. Indirect plate ELISA was able to demonstrate the antibody titer at different weeks post infection in experimental goats. A comparison of plate ELISA on suspected field sera and faecal sample examination by floatation method revealed that 68 (34.34%) samples were found to be positive using ELISA and twelve by faecal examination. The highest prevalence was found in lower age groups in both the seasons, with the increase in age, the infection level decreased. The prevalence was higher in wet season as compared to dry season. The results were stastically significant (P < 0.05). No sex predisposition was seen. Sensitivity and specificity of ELISA was 80.0%, and 21.42%, respectively. This test is therefore quite sensitive for clinical cases; an early diagnosis, however lacks specificity of faecal examination were 10.81% and 90.0%, respectively. [Vet. World 2011; 4(11.000): 503-506] |
| Databáze: | Directory of Open Access Journals |
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