Sperm pretreatment with glutathione improves IVF embryos development through increasing the viability and antioxidative capacity of sex-sorted and unsorted bull semen

Autor: Ting-xi HU, Hua-bin ZHU, Wei-jun SUN, Hai-sheng HAO, Xue-ming ZHAO, Wei-hua DU, Zong-li WANG
Jazyk: angličtina
Rok vydání: 2016
Předmět:
Zdroj: Journal of Integrative Agriculture, Vol 15, Iss 10, Pp 2326-2335 (2016)
Druh dokumentu: article
ISSN: 2095-3119
43274420
DOI: 10.1016/S2095-3119(16)61402-8
Popis: The antioxidant of reduced glutathione (GSH) is the most abundant thiol in cells for the maintenance of the intracellular redox balance. The study aimed to assay the effect of sperm treatment with GSH before incubation with oocytes on the development potential of embryos obtained by in vitro fertilization (IVF). Also the mitochondrial membrane potential (ΔΦm), plasma membrane integrity (viability), DNA fragmentation, reactive oxygen species (ROS) content, superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GPx) activities, methane dicarboxylic aldehyde (MDA) level as indices of lipid peroxidation in sex-sorted and unsorted sperm from three bulls were investigated using flow cytometry and enzyme-labeled instrument individually. The results showed that 2 mmol L−1 GSH increased significantly the cleavage rate (86.68% vs. 82.78%), 4- to 8-cell rate (82.30% vs. 73.43%) and blastocyst rate (43.15% vs. 35.24%) of IVF embryos compared with untreated group. Furthermore, addition of GSH increased significantly the ΔΦm and viability, decreased the ratio of DNA fragmentation in sex-sorted or unsorted semen (P
Databáze: Directory of Open Access Journals
Externí odkaz: