Effect of Incubation Time and Vitamin E Supplementation on Sperm Motility, Viability and DNA Fragmentation in Asthenoteratozoospermic Samples
| Autor: | Ali Asghar Ghafarizadeh, Gholamhassan Vaezi, Seyed Mohammad Ali Shariatzadeh, Ali Akbar Malekirad |
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| Jazyk: | perština |
| Rok vydání: | 2017 |
| Předmět: | |
| Zdroj: | Majallah-i dānishgāh-i ̒ulūm-i pizishkī-i Arāk, Vol 20, Iss 6, Pp 62-72 (2017) |
| Druh dokumentu: | article |
| ISSN: | 1735-5338 2008-644X |
| Popis: | Abstract Background: In Asthenoteratozoospermic men, low motility, defected DNA and highly oxidative stress in sperm cause poor assisted reproductive techniques (ART) outcomes. The aim of this study was to determine the effect of Vitamin E (Vit E), as a potent antioxidant, on sperm motility, viability and DNA integrity at different times of in vitro incubation (after 2, 4 and 6-h) to improve asthenoteratozoospermic semen samples for ART. Materials and Methods: Asthenoteratozoospermic semen samples of 50 volunteers were collected and examined. Each sample was divided into two groups of control and vitamin E (2mM) and kept in the 37 °C and 6 % CO2 for 2, 4 and 6 hours. After this incubation, sperm motility, viability and sperm DNA fragmentation (SCD) were evaluated in each group. Data were analyzed using repeated measurement of ANOVA and T-test. The means were considered significantly different at p |
| Databáze: | Directory of Open Access Journals |
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