In Vivo and In Vitro Toxicity Profiles of Hexane Extract of Alpinia malaccensis Rhizome in Rat and Cell Line Models

Autor: T. Somarathna, M. G. Thammitiyagodage, K. K. D. S. Ranaweera, G. A. S. Premakumara, M. A. Akbarsha, B. Kadalmani, N. S. Weerakkody
Jazyk: angličtina
Rok vydání: 2021
Předmět:
Zdroj: Journal of Toxicology, Vol 2021 (2021)
Druh dokumentu: article
ISSN: 1687-8191
1687-8205
DOI: 10.1155/2021/9578474
Popis: The objective of the study was to evaluate the potential toxicity of crude n-hexane extract of Alpinia malaccensis rhizome. The in vivo acute oral toxicity was evaluated by administering a single oral dose of the extract at 0, 300, or 2000 mg/kg body weight to female Wistar rats according to modified OECD Test Guideline 423. For the in vitro cytotoxicity study, A549, HepG2, 3T3, and COS-7 cell lines were exposed to different doses of A. malaccensis extract and cell viability was assessed adopting MTT assay followed by AO/EB staining, Hoechst staining, and comet assay with a view to compare the cellular and molecular mechanisms underlying the toxicity, if any. It was found that administration of 2000 mg/kg bw dose in in vivo oral acute toxicity study did not produce significant toxicity or mortality. No significant (p
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