Effect of light polariztion on pattern illumination super-resolution imaging
| Autor: | Caimin Qiu, Jianling Chen, Zexian Hou, Chaoxian Xu, Shusen Xie, Hongqin Yang |
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| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: | |
| Zdroj: | Journal of Innovative Optical Health Sciences, Vol 9, Iss 3, Pp 1641001-1-1641001-8 (2016) |
| Druh dokumentu: | article |
| ISSN: | 1793-5458 1793-7205 17935458 |
| DOI: | 10.1142/S1793545816410017 |
| Popis: | Far-field fluorescence microscopy has made great progress in the spatial resolution, limited by light diffraction, since the super-resolution imaging technology appeared. And stimulated emission depletion (STED) microscopy and structured illumination microscopy (SIM) can be grouped into one class of the super-resolution imaging technology, which use pattern illumination strategy to circumvent the diffraction limit. We simulated the images of the beads of SIM imaging, the intensity distribution of STED excitation light and depletion light in order to observe effects of the polarized light on imaging quality. Compared to fixed linear polarization, circularly polarized light is more suitable for SIM on reconstructed image. And right-handed circular polarization (CP) light is more appropriate for both the excitation and depletion light in STED system. Therefore the right-handed CP light would be the best candidate when the SIM and STED are combined into one microscope. Good understanding of the polarization will provide a reference for the patterned illumination experiment to achieve better resolution and better image quality. |
| Databáze: | Directory of Open Access Journals |
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