| Autor: |
Welsh Gavin I, Leney Sophie E, Pettitt Trevor R, Heyward Catherine A, Tavaré Jeremy M, Wakelam Michael JO |
| Jazyk: |
angličtina |
| Rok vydání: |
2008 |
| Předmět: |
|
| Zdroj: |
BMC Cell Biology, Vol 9, Iss 1, p 25 (2008) |
| Druh dokumentu: |
article |
| ISSN: |
1471-2121 |
| DOI: |
10.1186/1471-2121-9-25 |
| Popis: |
Abstract Background Insulin stimulates glucose uptake by adipocytes through increasing translocation of the glucose transporter GLUT4 from an intracellular compartment to the plasma membrane. Fusion of GLUT4-containing vesicles at the cell surface is thought to involve phospholipase D activity, generating the signalling lipid phosphatidic acid, although the mechanism of action is not yet clear. Results Here we report the identification of a putative phosphatidic acid-binding motif in a GLUT4 intracellular loop. Mutation of this motif causes a decrease in the insulin-induced exposure of GLUT4 at the cell surface of 3T3-L1 adipocytes via an effect on vesicle fusion. Conclusion The potential phosphatidic acid-binding motif identified in this study is unique to GLUT4 among the sugar transporters, therefore this motif may provide a unique mechanism for regulating insulin-induced translocation by phospholipase D signalling. |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
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