Pepper Mild Mottle Virus Coat Protein Alone Can Elicit the Capsicum spp. L3 Gene-Mediated Resistance

Autor: P. Gilardi, I. García-Luque, M. T. Serra
Jazyk: angličtina
Rok vydání: 1998
Předmět:
Zdroj: Molecular Plant-Microbe Interactions, Vol 11, Iss 12, Pp 1253-1257 (1998)
Druh dokumentu: article
ISSN: 1943-7706
0894-0282
DOI: 10.1094/MPMI.1998.11.12.1253
Popis: The pepper mild mottle virus (PMMoV-S) (an L3 hypersensitive response [HR]-inducer strain) coat protein was expressed in Capsicum chinense (L3L3) plants with the heterologous potato virus X (PVX)-based expression system. The chimeric virus was localized in the inoculated leaves and induced the HR, thus indicating that the tobamoviral sequences that affect induction of the HR conferred by the L3 resistance gene reside in the coat protein gene. Furthermore, transient expression of the PMMoV-S coat protein in C. chinense leaves by biolistic co-bombardment with a plasmid expressing the β-glucuronidase (GUS) gene leads to the induction of cell death and expression of host defense genes. Thus, the coat protein of PMMoV-S is the elicitor of the Capsicum spp. L3 resistance gene-mediated HR.
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