Development of HA/Ag-NPs Composite Coating from Green Process for Hip Applications
| Autor: | Denisse A. Lozoya-Rodríguez, Renata de Lima, Leonardo F. Fraceto, Antonio Ledezma Pérez, Mercedes Bazaldua Domínguez, Roberto Gómez Batres, Armando Reyes Rojas, Víctor Orozco Carmona |
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| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: | |
| Zdroj: | Molecules, Vol 22, Iss 8, p 1291 (2017) |
| Druh dokumentu: | article |
| ISSN: | 1420-3049 22081291 |
| DOI: | 10.3390/molecules22081291 |
| Popis: | In the present study, biological hydroxyapatite (HA) was obtained from bovine bones through a thermal process. A total of 0% and 1% of silver nanoparticles (Ag-NPs) synthesized from Opuntia ficus (nopal) were added to the biological hydroxyapatite coatings using an atmospheric plasma spray (APS) on a Ti6Al4V substrate. Following this, its antimicrobial efficiency was evaluated against the following bacterial strains: Escherichia coli, Staphylococcus aureus, and Pseudomonas aeruginosa. This was conducted according to the Japanese Industrial Standard (JIS) Z2801:2000 “Antimicrobial Product-Test for Antimicrobial Activity and Efficacy”. Scanning electron microscopy (SEM) showed that the silver nanoparticles (Ag-NPs) were evenly distributed on the coating surface. Energy dispersive X-ray spectroscopy (EDX) shows that apatite deposition occurs on a daily basis, maintaining a Ca/P rate between 2.12 and 1.45. Biocompatibility properties were evaluated with osteoblast-like cells (MC3T3-E1) by single-cell gel electrophoresis assay and Tali image cytometry. |
| Databáze: | Directory of Open Access Journals |
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