Analysis of an antioxidative defence system of hydrogen peroxide-treated pancreatic islet-derived 1.1B4 cells and siRNA targeting NR4A3-treated cells by microarray

Autor: Motoko Nakayama, Etsuko Ueta, Mitsuru Yoshida, Yuri Shimizu, Atsuko Tokuda, Yasuko Sone, Yuri Nomi, Y. Otsuka
Jazyk: angličtina
Rok vydání: 2023
Předmět:
Zdroj: Redox Report, Vol 28, Iss 1 (2023)
Druh dokumentu: article
ISSN: 13510002
1743-2928
1351-0002
DOI: 10.1080/13510002.2023.2247150
Popis: ABSTRACTPancreatic islet β-cells weaken under oxidative stress. In this study, human pancreatic islet-derived 1.1B4 cells were exposed to H2O2 and analysed using a human microarray, which revealed that heme oxygenase 1 (HMOX1), glutamate-cysteine ligase, early growth response 1, nuclear receptor subfamily 4 group A member 3 (NR4A3) and jun B proto-oncogene were upregulated, whereas superoxide dismutase 1 and catalase were not. Expression of NR4A3 rapidly increased after H2O2 addition, and the 1.1B4 cells treated with siRNA targeting NR4A3 became sensitive to H2O2; further, HMOX1 expression was strongly inhibited, suggesting that NR4A3 is an oxidative stress-responsive transcription factor that functions through HMOX1 expression in pancreatic islet β-cells. Expression of cyclin E1 and cyclin-dependent kinase 1 was also inhibited by siRNAs targeting NR4A3.
Databáze: Directory of Open Access Journals
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