Minutes-timescale 3D isotropic imaging of entire organs at subcellular resolution by content-aware compressed-sensing light-sheet microscopy

Autor: Chunyu Fang, Tingting Yu, Tingting Chu, Wenyang Feng, Fang Zhao, Xuechun Wang, Yujie Huang, Yusha Li, Peng Wan, Wei Mei, Dan Zhu, Peng Fei
Jazyk: angličtina
Rok vydání: 2021
Předmět:
Zdroj: Nature Communications, Vol 12, Iss 1, Pp 1-13 (2021)
Druh dokumentu: article
ISSN: 2041-1723
DOI: 10.1038/s41467-020-20329-3
Popis: High resolution imaging of large biological volumes typically takes a long time from hours to days. Here the authors use a Bessel light-sheet approach combined with a content-aware compressed sensing computational pipeline to image whole mouse organs at subcellular resolution in a few minutes.
Databáze: Directory of Open Access Journals