Autor: |
Mark C Harris, Dillon Cislo, Joan S Lenz, Christopher Umbach, Manfred Lindau |
Jazyk: |
angličtina |
Rok vydání: |
2017 |
Předmět: |
|
Zdroj: |
PLoS ONE, Vol 12, Iss 3, p e0173993 (2017) |
Druh dokumentu: |
article |
ISSN: |
1932-6203 |
DOI: |
10.1371/journal.pone.0173993 |
Popis: |
Although several proteins have been implicated in secretory vesicle tethering, the identity and mechanical properties of the components forming the physical vesicle-plasma membrane link remain unknown. Here we present the first experimental measurements of nanomechanical properties of secretory vesicle-plasma membrane tethers using combined AFM force clamp and TIRF microscopy on membrane sheets from PC12 cells expressing the vesicle marker ANF-eGFP. Application of pulling forces generated tether extensions composed of multiple steps with variable length. The frequency of short ( |
Databáze: |
Directory of Open Access Journals |
Externí odkaz: |
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