| Autor: |
Fatemeh Alipour, Mehdi Jalali, Mohammad Reza Nikravesh, Alireza Fazel, Mojtaba Sankian, Elnaz Khordad |
| Jazyk: |
angličtina |
| Rok vydání: |
2022 |
| Předmět: |
|
| Zdroj: |
Crescent Journal of Medical and Biological Sciences, Vol 9, Iss 1, Pp 9-18 (2022) |
| Druh dokumentu: |
article |
| ISSN: |
2148-9696 |
| DOI: |
10.34172/cjmb.2022.03 |
| Popis: |
Objectives: Dysfunction of the thyroid gland has a negative effect on the male reproductive system. Studies also show that extracellular matrix (ECM) components play an essential role in testicular development and function. In hypothyroidism, there is a significant disruption in the ECM structure of mammalian tissues. In addition, notable changes have been reported in the germ cell population under a hypothyroid state. This study aimed to investigate the relationship between ECM proteins and apoptosis of testicular germ cells due to hypothyroidism. Materials and Methods: In the present experimental study, 20 male Balb/C mice were divided into control and hypothyroid groups. The hypothyroid group received 0.05% 6-n-propyl-2-thiouracil (PTU) through drinking water for 35 days. Finally, real-time polymerase chain reaction, immunohistochemistry, periodic acid-Schiff (PAS) staining, terminal transferase-mediated dUTP nick-end labelling (TUNEL) assay, and biochemical measurements were performed after hypothyroidism confirmation. Results: Laminin α5 and collagen IV mRNA levels were upregulated in the hypothyroid group compared to the controls (P |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
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