Molecular surveillance of the Newcastle disease virus in domestic and wild birds on the North Eastern Coast and Amazon biome of Brazil

Autor: LM Thomazelli, J de Araujo, C de S Ferreira, R Hurtado, DB Oliveira, T Ometto, M Golono, L Sanfilippo, C Demetrio, ML Figueiredo, EL Durigon
Jazyk: angličtina
Rok vydání: 2012
Předmět:
Zdroj: Brazilian Journal of Poultry Science, Vol 14, Iss 1, Pp 01-07 (2012)
Druh dokumentu: article
ISSN: 1516-635X
1806-9061
DOI: 10.1590/S1516-635X2012000100001
Popis: Brazil is one of the world's largest countries with a rich diversity of wildlife, including resident and migratory wild birds, which may be natural reservoirs of the Newcastle disease virus (NDV). Because Brazil is a major global exporter of chicken meat, the emergence of such a disease may have a huge negative impact not only on the economy due to trade restrictions and embargoes, but also on the quality of life of the population. Samples were collected from 1,022 asymptomatic domestic and wild birds from the Brazilian coast and the Amazon region using tracheal/cloacal swabs and tested by RT-qPCR. The results showed 7 (0.7%) birds were positive for NDV. The positive samples were then isolated in embryonated chicken eggs and their matrix protein genes were partially sequenced, revealing a low-pathogenicity NDV. This study confirms the maintenance of the velogenic-NDV free status of Brazil.
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