Protocol to preserve mouse freeze-dried spermatozoa in the thin plastic sheets

Autor: Daiyu Ito, Teruhiko Wakayama
Jazyk: angličtina
Rok vydání: 2021
Předmět:
Zdroj: STAR Protocols, Vol 2, Iss 4, Pp 100933- (2021)
Druh dokumentu: article
ISSN: 2666-1667
DOI: 10.1016/j.xpro.2021.100933
Popis: Summary: The preservation of mammalian freeze-dried (FD) spermatozoa is commonly performed using small glass ampules; however, they are bulky and breakable. In this study, we present a protocol to prepare and preserve mouse FD sperm using thin plastic sheets. This approach allows storing thousands of mouse strains in a card folder. We can also send the FD sperm domestically using a postcard without any extra equipment.For complete details on the use and execution of this protocol, please refer to Ito et al. (2021).
Databáze: Directory of Open Access Journals