Biosensor libraries harness large classes of binding domains for construction of allosteric transcriptional regulators

Autor: Javier F. Juárez, Begoña Lecube-Azpeitia, Stuart L. Brown, Christopher D. Johnston, George M. Church
Jazyk: angličtina
Rok vydání: 2018
Předmět:
Zdroj: Nature Communications, Vol 9, Iss 1, Pp 1-12 (2018)
Druh dokumentu: article
ISSN: 2041-1723
DOI: 10.1038/s41467-018-05525-6
Popis: Bacterially encoded environmental sensor proteins are potentially a rich source of transcriptional control but only a few have been harnessed for biotechnological applications. Here the authors develop a general strategy for designing custom-made monogenic synthetic sensors and validate the approach by designing two sense-and-respond regulators for benzoate.
Databáze: Directory of Open Access Journals