| Autor: |
Kui Chen, Zhongwei Zhuang, Qi Wang, Keqin Li, Siyi Xu, Min Liu, Kuiming Zhang, Liang Wei, Yanfei Zhang, Hao Lian, Bei Zhang, Chunlong Zhong |
| Jazyk: |
angličtina |
| Rok vydání: |
2021 |
| Předmět: |
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| Zdroj: |
Stem Cell Research, Vol 56, Iss , Pp 102546- (2021) |
| Druh dokumentu: |
article |
| ISSN: |
1873-5061 |
| DOI: |
10.1016/j.scr.2021.102546 |
| Popis: |
Glioblastoma is the most common malignant primary brain tumor with poor prognosis and low long-term survival rate. Here we described the production of an iPSC line generated from peripheral blood mononuclear cells (PBMCs) of a 24-year-old female gliocytoma patient. The PBMCs were reprogrammed by the non-integrating Sendai Virus with human OKSM (OCT3/4, SOX2, KLF4, and c-MYC) transcription factors. The generated iPSCs were positive for pluripotent stem cell markers demonstrated by immunocytochemistry. Besides, they displayed a normal karyotype and had the potential to differentiate spontaneously three germ layers in vitro. Our model might be useful in studying the pathogenesis of gliocytoma patients. |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
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