Circulating ceramide levels and ratios in Emirati youth under 18 years: associations with cardiometabolic risk factors

Autor: Youssef M. Shalaby, Bashar Al-Zohily, Anjana Raj, Javed Yasin, Sania Al Hamad, Charalambos Antoniades, Nadia Akawi, Elhadi H. Aburawi
Jazyk: angličtina
Rok vydání: 2024
Předmět:
Zdroj: Lipids in Health and Disease, Vol 23, Iss 1, Pp 1-15 (2024)
Druh dokumentu: article
ISSN: 1476-511X
DOI: 10.1186/s12944-024-02080-6
Popis: Abstract Background Circulating ceramide (Cer) drives various pathological processes associated with cardiovascular diseases, liver illness, and diabetes mellitus. Although recognized as predictors of cardiometabolic diseases (CMD) in research and clinical settings, their potential for predicting CMD risk in individuals under 18 remains unexplored. Objectives This study was designed to utilize Liquid Chromatography-Mass Spectrometry (LC-MS/MS) methodology to determine the biological reference ranges for Cer in plasma samples of Emirati children and develop a risk assessment score (CERT-1) based on Cer concentrations. Methods Using LC-MS/MS, we developed a method to measure five Cer species in plasma samples of 582 Emirati participants aged 5–17. We used the circulating concentrations of these Cer to determine their reference intervals in this population. We employed traditional statistical analyses to develop a risk score (CERT-1) and assess the association between Cer levels and conventional biomarkers of CMD. Results We validated a high-throughput methodology using LC–MS/MS to quantify five Cer species in human plasma. Reference values for this population (n = 582) were quantified: CerC16:0 (0.12–0.29 µmol/L), CerC18:0 (0.019–0.067 µmol/L), CerC22:0 (0.102–0.525 µmol/L), CerC24:0 (0.65–1.54 µmol/L) and CerC24:1 (0.212–0.945 µmol/L). We devised a risk assessment score (CERT-1) based on plasma Cer content in the study participants, showing that 72.5% have low to moderate risk and 9.3% are at a higher risk of developing CMD. Our analyses also revealed a significant correlation (P
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