Probucol Protects Endothelial Progenitor Cells Against Oxidized Low-Density Lipoprotein via Suppression of Reactive Oxygen Species Formation In Vivo

Autor: Qingbin Zhang, Liming Chen, Zhihua Si, Haoran Bu, Chandrakala A. Narasimhulu, Xueling Song, Ming-Yu Cui, Hang Liu, Tiewei Lu, Guanglong He, Sampath Parthasarathy, Lianqun Cui, Zhenguo Liu, Yuqi Cui
Jazyk: angličtina
Rok vydání: 2016
Předmět:
Zdroj: Cellular Physiology and Biochemistry, Vol 39, Iss 1, Pp 89-101 (2016)
Druh dokumentu: article
ISSN: 1015-8987
1421-9778
DOI: 10.1159/000445608
Popis: Background/Aims: Oxidized low-density lipoprotein (ox-LDL) is a major component of hyperlipidemia and contributes to atherosclerosis. Endothelial progenitor cells (EPCs) play an important role in preventing atherosclerosis and notably decreased in hyperlipidemia. Ox-LDL and ox-LDL-related reactive oxygen species (ROS) have deleterious effects on EPCs. Probucol as an antioxidant and anti-inflammatory drug reduces ROS production. The present study was to determine if probucol could protect EPCs from ox-LDL in vivo and to investigate the potential mechanisms. Methods: ox-LDL was injected into male C57BL/6 mice for 3 days with or without probucol treatment with PBS as control. Bone marrow (BM) fluid, serum, circulating mononuclear cells (MNCs) and EPCs were collected for analysis. Results: the increased extracellular ROS in BM, serum and blood intracellular ROS production in the mice with ox-LDL treatment in association with a significant reduction of circulating MNCs and EPCs were restored with Probucol treatment. A significant increase in the serum ox-LDL and C-reactive protein and decrease in superoxide dismutase and circulating MNCs and EPCs were observed in hyperlipidemic patients that were effectively reversed with probucol treatment. Conclusion: these data suggested that probucol could protect EPCs from ox-LDL through inhibition of ROS production in vivo.
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