Analysis of Ct value of HBV nucleic acid detection in different blood donors
| Autor: | Jingwen CHEN, Shaobin CHEN, Runkui WEI, Qiuting YUAN, Ziyi HE |
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| Jazyk: | čínština |
| Rok vydání: | 2023 |
| Předmět: | |
| Zdroj: | Zhongguo shuxue zazhi, Vol 36, Iss 10, Pp 924-927 (2023) |
| Druh dokumentu: | article |
| ISSN: | 1004-549X 1004-549x |
| DOI: | 10.13303/j.cjbt.issn.1004-549x.2023.10.015&lang=en |
| Popis: | Objective To analyze the difference of Ct value of HBsAg-/HBV DNA + in blood samples from different types of voluntary blood donors by double ELISA and HBV DNA (MP6) detection, and to investigate the correlation between Ct value and the frequency of repeated blood donation, the first nucleic acid reactivity and the interval time of previous blood donation, so as to provide reference for laboratory evaluation of the effectiveness of nucleic acid testing(NAT) strategy for repeated blood donors occult hepatitis B virus infection(OBI). Methods The Ct value and information of blood donors from February 2019 to January 2022 in our laboratory were collected. According to the cumulative number of blood donations, they were divided into two groups:first-time blood donor group (Group A) and repeated blood donor group (Group B). Group B was subdivided into Group C 1( twice of blood donation) and group C 2(three or more times of blood donation) according to the cumulative times of blood donation, and Group D 1(< 1 year), Group D 2(1-3 years), Group D 3(3 years or more) according to the first NAT reactivity and the time of previous blood donation, the difference of Ct value and resolution yeild of HBV DNA in each group was compared. The yeild of HBV DNA in two groups was compared by chi-square test, and the difference of Ct values were compared by Nonparametric test. Results From February 2019 to January 2022, a total of 270 283 blood donors were tested, including 135 695 in Group A and 134 588 in Group B. The yeild of HBV DNA in Group A was 0.150% (203/135 695), which was higher than that in Group B [0.083% (111/134 588)] (P |
| Databáze: | Directory of Open Access Journals |
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