| Autor: |
Paiboon Jitprasertwong, Niratcha Chaisomboon, Kusuma Jamdee |
| Jazyk: |
angličtina |
| Rok vydání: |
2013 |
| Předmět: |
|
| Zdroj: |
Journal of Dental Sciences, Vol 8, Iss 4, Pp 358-364 (2013) |
| Druh dokumentu: |
article |
| ISSN: |
1991-7902 |
| DOI: |
10.1016/j.jds.2013.07.001 |
| Popis: |
Background/purpose: Progesterone and estrogen levels are elevated during pregnancy and play a role in maternal immune responses. In addition, unbalanced metabolism of growth factors has been demonstrated in pregnancy tumors. Therefore, we aimed to investigate the effect of progesterone and β-estradiol on vascular endothelial growth factor-A (VEGF-A) and basic fibroblast growth factor (bFGF) messenger RNA (mRNA) expression in THP-1 monocytes in response to Porphyromonas gingivalis lipopolysaccharide (LPS). Materials and methods: THP-1 monocytes were incubated with progesterone, β-estradiol, or LPS from Escherichia coli and P. gingivalis for up to 24 hours. The expression of VEGF-A and bFGF was investigated using conventional reverse transcription polymerase chain reaction (RT-PCR) and real-time RT-PCR. Cell growth was assessed using cell proliferation assay. Results: We reported herein that progesterone, but not β-estradiol, increased VEGF-A mRNA expression in THP-1 monocytes. Significantly, progesterone enhanced VEGF-A mRNA expression in P. gingivalis LPS-treated monocytes in comparison with a treatment with P. gingivalis LPS alone. However, neither β-estradiol nor progesterone had any effect on bFGF production at mRNA levels. Conclusion: The enhancing effect of progesterone on VEGF-A mRNA expression may have a role in the pathogenesis of pyogenic granuloma in pregnant women. |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
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