| Autor: |
Shin Yabukami, Toru Murayama, Koki Kaneko, Junichi Honda, Loi Tonthat, Kazuhiko Okita |
| Jazyk: |
angličtina |
| Rok vydání: |
2024 |
| Předmět: |
|
| Zdroj: |
AIP Advances, Vol 14, Iss 3, Pp 035102-035102-6 (2024) |
| Druh dokumentu: |
article |
| ISSN: |
2158-3226 |
| DOI: |
10.1063/9.0000836 |
| Popis: |
A quick and easy method to detect the GDF-15 protein (Growth Differentiation Factor-15) has been developed, which utilize the magnetic response of magnetic nanoparticles by switching magnetic fields. The magnetic nanoparticles and GDF-15 are bound by an antigen-antibody reaction and aggregated into a spherical shape using a needle-shaped magnetic yoke. The density of GDF-15 changed as follows: 0, 1 ng/ml, 10 ng/ml, and 100 ng/ml. The increase of GDF-15 aggregated the magnetic nanoparticles and enhanced the signal-to-noise ratio. We also tried the sandwich-type bound method using a primary and secondary antibody with additional magnetic nanoparticles and obtained the enhancement of the magnetic signal in a lower concentration (under 10 ng/ml) of GDF-15. The cross-bridges between magnetic nanoparticle and the protein may strengthen the magnetic couplings of nanoparticles. |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
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