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Background Long-term adaptations and ongoing training seem to modify the energy system contribution in highly trained individuals. We aimed to compare the energy metabolism profile during sprint exercise in athletes of different specialties. Methods Endurance (n = 17, 20.3 ± 6.0 yrs), speed-power (n = 14, 20.3 ± 2.5 yrs), and mixed (n = 19, 23.4 ± 4.8 yrs) athletes performed adapted 15-second all-out test before and after a general preparation training period. The contribution of phosphagen, glycolytic, and aerobic systems was calculated using the three-component PCr-LA-O2 method. Results Between-group differences were observed in the contribution of energy systems in the first and second examinations. The proportions were 47:41:12 in endurance, 35:57:8 in team sports, and 45:48:7 in speed-power athletes. Endurance athletes differed in the phosphagen (p |