| Autor: |
Min Min Than, Jetsada Ruangsuriya, Chairat Uthaipibull, Somdet Srichairatanakool |
| Jazyk: |
angličtina |
| Rok vydání: |
2018 |
| Předmět: |
|
| Zdroj: |
Asian Pacific Journal of Tropical Biomedicine, Vol 8, Iss 7, Pp 360-364 (2018) |
| Druh dokumentu: |
article |
| ISSN: |
2221-1691 |
| DOI: |
10.4103/2221-1691.237079 |
| Popis: |
Objective: To produce fluorescent tagged recombinant erythroferrone protein (ERFE_eGFP) for laboratory investigations. Methods: Erythroferrone (ERFE) gene was fused to green fluorescent protein (eGFP) gene and cloned in a pSecTag2Hygro plasmid. The constructed plasmid was amplified in Escherichia coli DH5 α and the eGFP-fused ERFE (ERFE_eGFP) protein was expressed in human embryonic kidney (HEK293T) cell line. Results: The plasmid constructed from colony C6 contained ERFE_eGFP with the correct restriction sizes of 4.2 kb and expressed secretory ERFE_eGFP fusion protein (approximately size of 75 kDa) in HEK293T cell line. Conclusions: ERFE_eGFP recombinant protein is successfully expressed as a secretory functional protein and could be sensitively detected using fluorometry. This fusion protein might benefit future applications for localization of cellular ERFE receptors and competitive immunoassay of ERFE concentration. |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
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