Perfluorochemical Liquid-Adenovirus Suspensions Enhance Gene Delivery to the Distal Lung

Autor: Jeffrey A. Kazzaz, Marlene S. Strayer, Jichuan Wu, Daniel J. Malone, Hshi-chi Koo, Thomas H. Shaffer, Jonathan M. Davis, David S. Strayer, Marla R. Wolfson
Jazyk: angličtina
Rok vydání: 2011
Předmět:
Zdroj: Pulmonary Medicine, Vol 2011 (2011)
Druh dokumentu: article
ISSN: 2090-1836
2090-1844
DOI: 10.1155/2011/918036
Popis: We compared lung delivery methods of recombinant adenovirus (rAd): (1) rAd suspended in saline, (2) rAd suspended in saline followed by a pulse-chase of a perfluorochemical (PFC) liquid mixture, and (3) a PFC-rAd suspension. Cell uptake, distribution, and temporal expression of rAd were examined using A549 cells, a murine model using luciferase bioluminescence, and histological analyses. Relative to saline, a 4X increase in transduction efficiency was observed in A549 cells exposed to PFC-rAd for 2–4 h. rAd transgene expression was improved in alveolar epithelial cells, and the level and distribution of luciferase expression when delivered in PFC-rAd suspensions consistently peaked at 24 h. These results demonstrate that PFC-rAd suspensions improve distribution and enhance rAd-mediated gene expression which has important implications in improving lung function by gene therapy.
Databáze: Directory of Open Access Journals
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