| Autor: |
Daisuke Kamiya, Nana Takenaka-Ninagawa, Souta Motoike, Mikihito Kajiya, Teppei Akaboshi, Chengzhu Zhao, Mitsuaki Shibata, Sho Senda, Yayoi Toyooka, Hidetoshi Sakurai, Hidemi Kurihara, Makoto Ikeya |
| Jazyk: |
angličtina |
| Rok vydání: |
2022 |
| Předmět: |
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| Zdroj: |
npj Regenerative Medicine, Vol 7, Iss 1, Pp 1-17 (2022) |
| Druh dokumentu: |
article |
| ISSN: |
2057-3995 |
| DOI: |
10.1038/s41536-022-00241-8 |
| Popis: |
Abstract Mesenchymal stem/stromal cells (MSCs) are adult multipotent stem cells. Here, we induced MSCs from human induced pluripotent stem cells (iPSCs) via a neural crest cell (NCC) lineage under xeno-free conditions and evaluated their in vivo functions. We modified a previous MSC induction method to work under xeno-free conditions. Bovine serum albumin-containing NCC induction medium and fetal bovine serum-containing MSC induction medium were replaced with xeno-free medium. Through our optimized method, iPSCs differentiated into MSCs with high efficiency. To evaluate their in vivo activities, we transplanted the xeno-free-induced MSCs (XF-iMSCs) into mouse models for bone and skeletal muscle regeneration and confirmed their regenerative potency. These XF-iMSCs mainly promoted the regeneration of surrounding host cells, suggesting that they secrete soluble factors into affected regions. We also found that the peroxidasin and IGF2 secreted by the XF-iMSCs partially contributed to myotube differentiation. These results suggest that XF-iMSCs are important for future applications in regenerative medicine. |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
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