Fluorescent fullerene nanoparticle-based lateral flow immunochromatographic assay for rapid quantitative detection of C-reactive protein

Autor: Kyung Mi Park, Da Jung Chung, Mijin Choi, Taejoon Kang, Jinyoung Jeong
Jazyk: angličtina
Rok vydání: 2019
Předmět:
Zdroj: Nano Convergence, Vol 6, Iss 1, Pp 1-6 (2019)
Druh dokumentu: article
ISSN: 2196-5404
11577010
DOI: 10.1186/s40580-019-0207-0
Popis: Abstract A fluorescent fullerene nanoparticle (NP)-based lateral flow immunochromatographic assay (LFIA) was developed for the rapid and quantitative detection of C-reactive protein (CRP) in serum. The polyclonal CRP-antibody-conjugated fullerene NPs were simply prepared by 1-ethyl-3-(3-dimethyllaminopropyl)-carbodiimide hydrochloride coupling after carboxylation of fluorescent fullerene NPs. By applying the CRP-antibody-conjugated fullerene NPs to a lateral flow test strip, quantitative analysis of CRP in serum was possible at a concentration range of 0.1–10 ng/ml within 15 min. We anticipate that this novel fluorescent fullerene NP-based LFIA can be useful for the rapid and accurate sensing of biological and chemical species, contributing to the disease diagnosis and prognosis, environmental monitoring, and food safety.
Databáze: Directory of Open Access Journals
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