| Autor: |
Sviatlana Shashkova, Adam Wollman, Stefan Hohmann, Mark C Leake |
| Jazyk: |
angličtina |
| Rok vydání: |
2018 |
| Předmět: |
|
| Zdroj: |
Bio-Protocol, Vol 8, Iss 2 (2018) |
| Druh dokumentu: |
article |
| ISSN: |
2331-8325 |
| DOI: |
10.21769/BioProtoc.2710 |
| Popis: |
Single-molecule fluorescence microscopy enables unrivaled sub-cellular quantitation of genomically encoded fusions of native proteins with fluorescent protein reporters. Fluorescent proteins must undergo in vivo maturation after expression before they become photoactive. Maturation effects must be quantified during single-molecule analysis. Here we present a method to characterise maturation of GFP and mCherry genetic protein fusions in budding yeast Saccharomyces cerevisiae. |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
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