Bacterial porin disrupts mitochondrial membrane potential and sensitizes host cells to apoptosis.
| Autor: | Vera Kozjak-Pavlovic, Elke A Dian-Lothrop, Michael Meinecke, Oliver Kepp, Katharina Ross, Krishnaraj Rajalingam, Anke Harsman, Eva Hauf, Volker Brinkmann, Dirk Günther, Ines Herrmann, Robert Hurwitz, Joachim Rassow, Richard Wagner, Thomas Rudel |
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| Jazyk: | angličtina |
| Rok vydání: | 2009 |
| Předmět: | |
| Zdroj: | PLoS Pathogens, Vol 5, Iss 10, p e1000629 (2009) |
| Druh dokumentu: | article |
| ISSN: | 1553-7366 1553-7374 |
| DOI: | 10.1371/journal.ppat.1000629 |
| Popis: | The bacterial PorB porin, an ATP-binding beta-barrel protein of pathogenic Neisseria gonorrhoeae, triggers host cell apoptosis by an unknown mechanism. PorB is targeted to and imported by host cell mitochondria, causing the breakdown of the mitochondrial membrane potential (DeltaPsi(m)). Here, we show that PorB induces the condensation of the mitochondrial matrix and the loss of cristae structures, sensitizing cells to the induction of apoptosis via signaling pathways activated by BH3-only proteins. PorB is imported into mitochondria through the general translocase TOM but, unexpectedly, is not recognized by the SAM sorting machinery, usually required for the assembly of beta-barrel proteins in the mitochondrial outer membrane. PorB integrates into the mitochondrial inner membrane, leading to the breakdown of DeltaPsi(m). The PorB channel is regulated by nucleotides and an isogenic PorB mutant defective in ATP-binding failed to induce DeltaPsi(m) loss and apoptosis, demonstrating that dissipation of DeltaPsi(m) is a requirement for cell death caused by neisserial infection. |
| Databáze: | Directory of Open Access Journals |
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