Molecular diversity of internal transcribed spacer among the monoconidial isolates of Magnaporthe oryzae isolated from rice in Southern Karnataka, India

Autor: D. Jagadeesh, M.K. Prasanna Kumar, R. Chandrakanth, N.S. Devaki
Jazyk: angličtina
Rok vydání: 2018
Předmět:
Zdroj: Journal of Genetic Engineering and Biotechnology, Vol 16, Iss 2, Pp 631-638 (2018)
Druh dokumentu: article
ISSN: 1687-157X
DOI: 10.1016/j.jgeb.2018.05.008
Popis: Blast disease of rice plant is caused by Magnaporthe oryzae (anamorph Pyricularia oryzae). This disease is recognized to be one of the most serious diseases of rice crop around the world. A total of 72 monoconidial isolates of M. oryzae obtained from blast disease samples collected around Southern Karnataka were characterized using internal transcribed spacers of the ribosomal DNA sequences. These were analyzed by comparing with already deposited sequences in GenBank database. It helped in diagnosing the invasive pathogen in all locations. Variability of rDNA sequences was found to be highly polymorphic with 0.068962 nucleotide diversity showing 6 distinct clades. 33 haplotype groups were identified with haplotype diversity of 0.8881 and Tajima's neutrality test with a D value of −1.96827 with P
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